Located in Upham Hall, room 100, on the main campus of Miami University (Oxford, Ohio), the Hefner Museum of Natural History uses exhibits, collections and specimens, and interactive programs to teach visitors of all ages about biology and natural history. Through its exhibits and collections, the Hefner Museum helps visitors to understand and appreciate the importance of biodiversity, conservation, and ecology.

Its primary audiences are Miami University students, faculty and staff, preK-12 students, teachers and parents/guardians, and the general public. The museum also supports scholarship, research, and educational projects.