Real-Time PCR

Quantitative polymerase chain reaction or qPCR, as well as reverse transcriptase qPCR (gene expression) RT-qPCR, are important applications supported by the CBFG. For these experiments, we use:

  • Qiagen Rotor-Gene Q 5-Plex HRM platform
  • Bio-Rad CFX Connect (96 well)
    • Capable of using five fluors (simultaneously) as well as High-Resolution Melt applications (taxa ID, epigenetics, mutation scanning)
    • Up to 100 samples concurrently
  • Thermo Fisher QuantStudio 7 Flex Real-Time PCR System
    • 96 or 384 well sample blocks
    • 6 colour multiplexing

These instruments are calibrated for most of the fluorophores that are commonly used for qPCR experiments.

Experimental design is critical to success in qPCR experiments. The CBFG staff provides training and consultation for all aspects of qPCR based on MIQE recommended guidelines, ranging from experimental design and instrument operation to data interpretation and validation to ensure your best results..