Proteomics

Proteomics is a complementary field to nucleic acid investigations supported by the CBFG. We have much of the preparatory equipment and protocols required to perform highly parallel, 2-dimensional gel electrophoresis, as well as prepare samples for mass spectrometry (MALDI and OrbiTrap).

A Bio-Rad ChemiDoc MP Imaging System for Stain-Free Western Blotting. This system can be used for imaging both 1-D and 2-D gels. This system can produce high-quality images with a very wide variety of staining methods including colourmetric, chemiluminescent and fluorescent stains. With four-fold log sensitivity, this is a flexible and versatile instrument.

ÄKTA FPLC (Fast Protein Liquid Chromatography) purification system from Cytiva Life Sciences offers users the ability to purify proteins from complex starting materials such as tissue homogenates. Routine and reproducible purifications with UV detection, dual pump system, a variety of CBFG maintained columns for SEC, Affinity, IEX, HISTrap, and other applications are available. Automatic fraction collection is also available, as is training on the UNICORN software.