Manager of Science Lab Operations

Levy Hall, 231
Middletown, OH 45042
513-727-3240
howartjw@MiamiOH.edu

Education

• Ph.D., Rice University, Biochemistry, 1992
• B.S. California University of Pennsylvania, Chemistry, 1982

Research Interests

The use of nuclear magnetic resonance (NMR) to solve biological and physiological problems. The use of NMR and MS-LC for biomarker identification.

Publications

Howarth, J., Krudy, G.A, Lin, X., Putkey, J.A. and Rosevear, P.R. (1993) Mapping of Methionine Residues in Cardiac Troponin C Upon Binding of Calcium, Troponin I Peptide and Bepridil, Fed. Proc. Am. Soc. Exp. Biol. 1993 7, A1078.

Howarth, J., Krudy, G.A., Lin., X. Putkey, J.A. and Rosevear, P.R. Mapping of Methionine Residues in Cardiac Troponin C Upon Binding Calcium and Troponin I Peptide. BioPhysical J. 1994 66, A123.

Krudy GA, Kleerekoper Q, Guo X, Howarth JW, Solaro RJ, Rosevear PR. “NMR studies delineating spatial relationships within the cardiac troponin I-troponin C complex.” J Biol Chem. 1994 Sep 23;269(38):23731-5.

Lin X, Krudy GA, Howarth J, Brito RM, Rosevear PR, Putkey JA. “Assignment and calcium dependence of methionyl epsilon C and epsilon H resonances in cardiac troponin C.” Biochemistry. 1994 Dec 6;33(48):14434-42.

Howarth JW, Krudy GA, Lin X, Putkey JA, Rosevear PR. “An NMR and spin label study of the effects of binding calcium and troponin I inhibitory peptide to cardiac troponin C.” Protein Sci. 1995 Apr;4(4):671-80.Kleerekoper Q,

Howarth JW, Guo X, Solaro RJ, Rosevear PR. “Cardiac troponin I induced conformational changes in cardiac troponin C as monitored by NMR using site-directed spin and isotope labeling.” Biochemistry. 1995 Oct 17;34(41):13343-52.

Gasmi-Seabrook GM, Howarth JW, Finley N, Abusamhadneh E, Gaponenko V, Brito RM, Solaro RJ, Rosevear PR. “Solution structures of the C-terminal domain of cardiac troponin C free and bound to the N-terminal domain of cardiac troponin I.” Biochemistry. 1999 Jun 29;38(26):8313-22. Erratum in: Biochemistry. 1999 Oct 26;38(43):14432.

Finley N, Abbott MB, Abusamhadneh E, Gaponenko V, Dong W, Gasmi-Seabrook G, Howarth JW, Rance M, Solaro RJ, Cheung HC, Rosevear PR. “NMR analysis of cardiac troponin C-troponin I complexes: effects of phosphorylation.” FEBS Lett. 1999 Jun 18;453(1- 2):107-12.

Gaponenko V, Howarth JW, Columbus L, Gasmi-Seabrook G, Yuan J, Hubbell WL, Rosevear PR. “Protein global fold determination using site-directed spin and isotope labeling.” Protein Sci. 2000 Feb;9(2):302-9.

Dvoretsky A, Abusamhadneh EM, Howarth JW, Rosevear PR. “Solution structure of calcium-saturated cardiac troponin C bound to cardiac troponin I.” J Biol Chem. 2002 Oct 11;277(41):38565-70. Epub 2002 Jul 29.

Finley NL, Howarth JW, Rosevear PR. “Structure of the Mg2+-loaded C-lobe of cardiac troponin C bound to the N-domain of cardiac troponin I: comparison with the Ca2+-loaded structure.” Biochemistry. 2004 Sep 14;43(36):11371-9.

Howarth, JW, Meller, J., Solaro, RJ, Trewhella, J, Rosevear, PR “Phosphorylation dependent conformational transition of the cardiac specific N-extension of troponin I in cardiac troponin” J. Mol. Biology 2007; 373: 706-722.

Sakthivel S, Finley NL, Howarth, JW, Osinska, H, Klevitsky, R., Lorenz JN, Rosevear, PR, Robbins J “Role of the acidic N’-region of cardiac troponin I in regulating myocardial function.” FASEB J. 2007; 22: 1246-1257.

Howarth, JW, Ramisetti, S, Nolan, K., Sadayappan, S, Rosevear, PR “Structural insight into the unique cardiac myosin binding protein-C motif: A partially folded domain.” J. Biol. Chem. 2012; 287(11): 8254-8262.

Michalek, A.J., Howarth, J.W., Gulick, J., Previs, M.J., Robbins, J., Rosevear, P.R. and Warshaw, D.M. “Phosphorylation modulates mechanical stability of the cardiac myosin binding protein-C motif.”, Biophysical J. 2013; 104(2) :442-452.